| http://www.w3.org/ns/prov#value | - The heating conditions necessary for denaturation will depend, e.g., on the buffer salt concentration and the length and nucleotide composition of the nucleic acids being denatured, but typically range from about 90??? to about 105??? C. for a time depending mainly on the temperature and the nucleic acid length, typically about 0.5 to four minutes.
|
