| http://www.w3.org/ns/prov#value | - Wavelengths nearer 225 nm are used since, although the peptide bond absorbs less strongly, the interference by oxygen is limited. +* '''In a typical protein solution used in enzymatic studies, the main interfering substances likely to affect absorbance measurements are nucleic acids, nucleotides such as ATP or NAD(P), haem-containing compounds such as cytochromes, reagents containing sulphydryl gr
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