| http://www.w3.org/ns/prov#value | - The column was then washed with 10 volumes of Buffer C, and then was eluted with a 2???500 ml gradient from 0.0 to 700 mM KCL in Buffer C. 10 ml fractions were collected throughout the procedure including loading, washing and eluant pass through, and were each fraction was assayed for DNA polymerase I activity as described herein.
|
