| http://www.w3.org/ns/prov#value | - Vacuum is then applied to the actuation chamber 313 to draw the fluid plugs through the porous fifth layer 305 into the mixing chamber 314, where the plugs mix. [0202] Another embodiment for mixing microfluidic plugs utilizes a porous material, preferably a hydrophobic porous material, to vent a mixing chamber or other reaction area.
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