| http://www.w3.org/ns/prov#value | - Typically, 500 mM EDTA is about 680 mM NaOH by the time you get it dissolved.I assume you are preparing 10 mM Tris-HCl, pH 8.0, 1 mM EDTA or 10 mM Tris-HCl, pH 7.6, 1 mM EDTA. Since Tris is a buffer, the pH of the Tris is the final pH. Prepare 1 M Tris-HCl at the desired pH. You must take into account the temperature sensitivity of Tris when preparing it.
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