| http://www.w3.org/ns/prov#value | - are you sure it is the plasmid toxic to cells? because as you reduce the amount of your plasmid, the amount of your transfection reagent should also be reduced. all kinds of reagents should be toxic and it is just a matter of dose. anyway, it is good that problem solved. -gyma- I like the freestyle max reagent for 293 cells, great system and low toxicity.
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