The complete set of 16 templates (7 circular and 9 linear) displayed significant mispriming at permissive conditions (permissive conditions include, for example, higher dNTP high concentrations, such as about 50 ??M or higher) and the mispriming was cleared at restrictive conditions of the SYN protocol (Table 15). (The SYN protocol here refers to the conditions in Table 13 under the heading Strata