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  • This example is a comparison of the inventive process (A and D in FIG. 3) with an independent COBRA method (See ???Methods,??? above) to determine the methylation status of a CpG island associated with the estrogen receptor (ESR1) gene in the human colorectal cell line HCT116 and in human sperm DNA. This CpG island has been reported to be highly methylated in HCT116 and unmethylated in human sperm
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