| http://www.w3.org/ns/prov#value | - Then the extracted methanol solution is distilled under reduced pressure at 100 millibar using water-bath at bath temperature of 100??C to get the crude shikimic acid. 20 ml of aqueous methanol (1 : 1) was added to the solid material containing mostly shikimic acid and the mixture was refluxed for 30 minutes, cooled to -10??C and maintained for 6 hours.
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