| http://www.w3.org/ns/prov#value | - I am facing a problem with protein purification by FPLC. ALL of a sudden since past six months all my protein preps seems to give me a weird type of sharp peak in the FPLC trace rite before my protein elutes and when i run the fraction on the gel there is no protein in there and other fractions seems to have protein which is pure but still i seem to get this peak all the time.
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